Seminar of the Division of Biomedical Physicochemistry
Microsoft Teams
Time-resolved & multiplexed FRET for advanced biosensing and bioimaging
prof. Niko Hildebrandt
Seoul National University & Université Paris-Saclay
Seminarium odbędzie się w trybie zdalnym przy użyciu programu Microsoft Teams. Osoby, które chcą wziąć udział w seminarium proszone są o kontakt pod adresem lub .
Streszczenie:
The investigation of biomolecular recognition via Förster Resonance Energy Transfer (FRET) plays an important role for quantifying concentrations and distances in many fields of the life sciences. Application of time-resolved photoluminescence spectroscopy and microscopy for the analysis of FRET offers several advantages concerning versatility, sensitivity, and specificity. The combination of nanoparticles (NPs), such as quantum dots (QDs), upconversion NPs (UCNPs), or gold NPs (AuNPs), molecular fluorophores, such as organic dyes or lanthanide complexes, and biological fluorophores, such as fluorescent proteins, provides almost unlimited possibilities for the design of advanced energy transfer systems. The same versatility applies for biological recognition molecules, including antibodies, aptamers, nucleic acids, and peptides. Therefore, the FRET-bioanalysis toolbox presents a boundless source for designing fluorescent probes for biosensing and bioimaging. The presentation will explain time-resolved and time-gated FRET and the specific benefits for spectral and temporal muminescence multiplexing with different materials and instruments. Then, recent applications concerning multiplexed FRET biosensing and bioimaging in solution, in-vitro, in-situ, and in-vivo will be discussed.
Recent reviews concerning our work: Accounts of Chemical Research 2022, 55(4), 551-564; Analytical Chemistry 2022, 94 (1), 193–224; TrAC – Trends in Analytical Chemistry 2020, 125, 115819; TrAC – Trends in Analytical Chemistry 2020, 123, 115748; Nature Methods 2019, 16 (9), 815-829; Expert Review of Molecular Diagnostics 2019, 19 (9), 767-771; ACS Sensors 2017, 2 (1), 31-45; Chemical Reviews 2017, 117 (2), 536-711.
