For students

Supervisor: prof. dr hab. Artur Bednarkiewicz
Auxillary supervisor: dr Małgorzata Misiak

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Description:

The rapid progress in molecular diagnostics, systems biology, and precision medicine creates a growing demand for novel biological and biochemical sensors characterized by extreme sensitivity, capable of detecting single-molecule events and ultra‑low analyte concentrations. One of the most promising approaches toward meeting these requirements is the exploitation of nonlinear photophysical processes, in particular avalanche photon emission and Förster Resonance Energy Transfer (FRET).

The objective of this PhD project is to develop and investigate new concepts of ultrasensitive optical sensors in which the detection signal is enhanced by avalanche photon emission processes coupled with FRET mechanisms between appropriately engineered energy donors and acceptors. The synergy between these two phenomena offers the potential for a substantial increase in signal‑to‑noise ratio, reduction of detection limits, and the realization of novel sensing functionalities.

The PhD research will include, in particular:

• design and synthesis of luminescent material systems (inorganic, organic, or hybrid) exhibiting avalanche photon emission,
• investigation of Förster Resonance Energy Transfer mechanisms in systems incorporating biological labels, biomolecules, or chemical probes,
• coupling of avalanche emission processes with FRET to achieve nonlinear amplification of sensing signals,
• optical, temporal, and spectral characterization of the developed systems,
• evaluation of sensitivity, selectivity, and stability of the sensors in biological and biochemical environments,
• proof‑of‑concept demonstrations of sensor performance in selected model applications (e.g., detection of proteins, nucleic acids, metabolites, or ions).

The project will employ advanced experimental and analytical techniques, including:

• absorption and luminescence spectroscopy (steady‑state and time‑resolved),
• characterization of nonlinear optical and avalanche emission processes,
• studies of FRET efficiency and excitation energy transfer kinetics,
• modeling of energy transfer processes and excitation dynamics,
• basic biofunctionalization techniques and preparation of biological samples.

The expected outcome of the project is the development of a new class of ultrasensitive optical sensors based on the synergy of avalanche photon emission and Förster Resonance Energy Transfer, together with an in‑depth understanding of the coupled photonic and biophysical mechanisms governing their operation. The results are anticipated to have high scientific impact and application potential in diagnostics, biosensing, and biomedical photonics.

Candidate profile

The ideal candidate should have:

• an MSc degree in physics, applied physics, optics, materials science, biophysics, or a related engineering discipline,
• basic knowledge in optics, electronics and software development (Matlab, LabView, Phyton)
• strong interest in photophysics, nanomaterials, biosensing, or optics,
• willingness to conduct experimental research and data analysis,
• motivation to work in an interdisciplinary research environment.